Table 3.
Primer sequences in fluorescence quantitative PCR reaction.
| Primer name | Sequences of the primer pair1 | Fragment length (bp) | GenBank no. |
|---|---|---|---|
| ACC | F:GCTGGGTTGAGCGACTAATG | 170 | NM_205505 |
| R:AAACTGGCAAAGGACTGACG | |||
| CPT-1α | F:GCCACTTATGAATGATGAGGAG | 174 | NM_001012898.1 |
| R:ATTATTGGTCCACGCCCTC | |||
| FAS | F:TGAAGGACCTTATCGCATTGC | 96 | NM_205155 |
| R:GCATGGGAAGCATTTTGTTGT | |||
| SREBP-1c | F:GTCGGCGATCCTGAGGAA | 105 | XM_046927256.1 |
| R:CTCTTCTGCACGGCCATCTT | |||
| PPARα | F:CAAACCAACCATCCTGACGAT | 64 | XM_046906390.1 |
| R:GGAGGTCAGCCATTTTTTGGA | |||
| AMPKα | F:GATATTTGGAGCAGTGGGGTTA | 248 | NM_001039603 |
| R:GGAAACAAGTATTTGGGAAGGT | |||
| ACO | F:TGCTGGTATTGAGGAATGTCG | 96 | NM_001006205.1 |
| R:CAGGATGGGGTGAACGTGA | |||
| LPL | F:TGGACAGATGGACAGCTTGG | 148 | NM_205282.1 |
| R:ATTTGAATCAGGTTCCCTCTTG | |||
| FABP | F:TCCAGAAGGGTAAGGACATCA | 224 | NM_204192.3 |
| R:TTGAGTTCGGTCACGGATTT | |||
| β-actin | F:TGCGTGACATCAAGGAGAAG | 300 | NM_205518.2 |
| R:TGCCAGGGTACATTGTGGTA |
1
The primers were designed using Primer Expression software Primer Premier 5.
Abbreviations: ACC, acetyl-coA carboxylase; ACO, lipoyl-coa oxidase; AMPKα, adenosine monophosphate-activated protein kinase; CPT-1α, carnitine palmityl transferase; FABPs, fatty acid binding protein; FAS, fatty acid synthase; LPL, lipoprotein decomposition enzyme; PPARα, peroxisome proliferator activated receptor; SREBP-1c, sterol regulatory element binding protein-1c.