. Author manuscript; available in PMC: 2024 May 18.

Published in final edited form as: Cell Chem Biol. 2023 May 5;30(5):457–469.e11. doi: 10.1016/j.chembiol.2023.04.006

Table 1.

Proteasome inhibition activity and Plasmodium growth inhibition of ATZs and their constituent pharmacophores

ID	IC₅₀ (nM)			EC₅₀ (nM)
ID	Pf20S	Hu i-20S	Hu c-20S	Pf Dd2^WT	Dd2β6^A117D	Dd2β5^A49S	HepG2

ART1	9,200 ± 720	>100,000	>100,000	43.7 ± 12.7	27.9 ± 11.0	30.2 ± 2.6	>100,000
PI01	6.0 ± 0.8	200 ± 46	2,000 ± 240	7.4 ± 0.2	121 ± 20	>2,770	>100,000
ATZ2	2.9 ± 0.6	73 ± 19	210 ± 40	3.0 ± 0.6	4.4 ± 1.1	10.9 ± 2.6	5,800 ± 620
ATZ4	4.3 ± 0.9	200 ± 57	890±140	4.3 ± 1.3	6.7 ± 2.2	5.9 ± 1.9	9,400 ± 1,700
Deoxy-ATZ4	4.4 ± 0.5	300 ± 22	1100 ± 46	21.6 ± 1.4	246.0 ± 44.5	>2,770	10,800 ± 500

All data are means of at least three independent experiments and are presented with standard deviation. Data were rounded to three or fewer significant figures.