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. Author manuscript; available in PMC: 2024 May 18.
Published in final edited form as: Cell Chem Biol. 2023 May 5;30(5):457–469.e11. doi: 10.1016/j.chembiol.2023.04.006

KEY RESOURCES TABLE

REAGENT or RESOURCE SOURCE IDENTIFIER
Chemicals, peptides, and recombinant proteins
PI01 This paper N/A
ART1 This paper N/A
ATZ2 This paper N/A
ATZ4 This paper N/A
Deoxy-ATZ4 This paper N/A
ATZ-P1 This paper N/A
Deoxy-ATZ-P1 This paper N/A
Artemisinin Sigma-Aldrich Catalog # 361593
Hemin Sigma-Aldrich Catalog #51280
Sodium ascorbate Sigma-Aldrich Catalog #PHR1279
Activity-based probe MV151 Verdoes et al.37 N/A
β2-specific inhibitor WLW-VS Li et al.29 N/A
Suc-LLVY-AMC Boston Biochem Catalog # S-280
Ac-ANW-AMC Boston Biochem Catalog # S-320
Trypsin Promega Catalog #V528A
Chymotrypsin Promega Catalog #V106A
Human constitutive proteasome Boston Biochem Catalog # E-360
Human 20S immunoproteasome Boston Biochem Catalog # E-370
Recombinant human PA28 activator alpha subunit Boston Biochem Catalog # E-381
P. falciparum 20S proteasome Kirkman et al.32 N/A
β-Casein Sigma-Aldrich Catalog #C6905
Bovine serum albumin Sigma-Aldrich Catalog #3117057001
12% Novex™ Bis-Tris Protein Gel ThermoFisher Scientific Catalog #NP0341BOX
MOPS SDS running buffer ThermoFisher Scientific Catalog # NP0001
Mini cOmplete EDTA-free Protease Inhibitor Cocktail Sigma-Aldrich Catalog# 11836170001
Critical commercial assays
Pierce BCA Protein Assay Kit ThermoFisher Scientific Catalog # 23225
CellTiter-Glo® Assay Kit Promega Catalog #G7573
Experimental models: Cell lines
HepG2 cell ATCC HB-8065™
Experimental models: Organisms/strains
Plasmodium falciparum Dd2 BEI Resources MRA-150
Plasmodium falciparum Cam3.1R539T BEI Resources MRA-1255
Plasmodium falciparum strain Cam3.1Rev BEI Resources MRA-1252
Plasmodium falciparum Dd2 β6A117D Kirkman et al.32 N/A
Plasmodium falciparum Dd2 βA49S Zhan et al.24 N/A
Plasmodium berghei ANKA Lux Franke-Fayard et al.53 N/A
Plasmodium berghei G2025R551T Simwela et al., 2021 N/A
Plasmodium berghei G1989M48SI Simwela et al., 2021 N/A
Plasmodium falciparum Dd2 β6A117DK13R539T This paper N/A
Swiss Webster (CFW) Mouse Charles River CFW(024)
Oligonucleotides
Single guide 1 sequence: ATGATCGTTTAAGAGATGTA This paper N/A
Single guide 2 sequence: ATACCTAGAAGAAATAATTG This paper N/A
Single guide 3 sequence: TGATCATCGTATGAAAGCAT This paper N/A
Repair template (Single guides are underlined and shield mutations are in bold) ATAGGTGGATTTGATGGTGTAGA ATATTTAAATTCGATGGAATTATTAGATATTAGTCAACAA TGCTGGCGTATGTGTACACCTATGTCTACCAAAAAAGC TTATTTTGGAAGTGCTGTATTGAATAATTTCTTATACGTT TTTGGTGGTAATAACTATGATTATAAGGCTTTATTTGAA ACTGAGGTGTATGATCGTTTACGTGACGTCTGGTATGT TTCAAGTAATTTAAATATACCTAGAAGAAACAACTGCGG TGTTACGTCAAATGGTACAATTTATTGTATTGGGGGATA TGATGGCTCTTCTATTATACCGAATGTAGAAGCATATGA TCATAGAATGAAGGCTTGGGTAGAGGTGGCACCTTTGA ATACCCCTAGATCATCAGCTATGTGTGTTGCTTTTGATA ATAAAATTTATGTCATTGGTGGAACTAATGGTGAGAGA TTAAATTCTATTGAAGTATATGAAGAAAAAATGAATAA ATGGGAACAATTTCCATATGCCTTATTAGAAGCT AGAAGTTCA This paper N/A
Software and algorithms
ImageJ Schneider et al.7 https://imagej.nih.gov/ij/
Chemdraw Pro PerkinElmer https://perkinelmerinformatics.com/products/research/chemdraw
GraphPad Prism 9 GraphPad Software Inc. https://www.graphpad.com/
Microsoft Office Suite Microsoft, Inc. N/A
Other
Slide-A-Lyzer MINI Dialysis Devices ThermoFisher Scientific Catalog # 88401