Skip to main content
. 2023 May 18;14(21):4914–4922. doi: 10.1021/acs.jpclett.3c00512

Figure 3.

Figure 3

Motility of chimera with the motor and lever arm domain from myosin X and the parallel coiled-coil domain from myosin V on actin filaments and actin bundles. (A) Schematic of chimera which contains the motor and lever arm domain from myosin X (green) and the parallel coiled-coil domain from myosin V (blue). (B) Representative traces of the chimera with the parallel coiled-coil domain moving on actin filaments (B1) and actin bundles (B2) at 2 μM ATP. (C) Step size histogram of the chimera with the parallel coiled-coil domain moving on actin filaments (C1) and actin bundles (C2) at 2 μM ATP. The step size histogram with the parallel coiled-coil domain on actin filaments was fitted the sum of 2 Gaussian components centered at −30.9 ± 15.5 and 40.3 ± 17.5 nm (n = 208). The step size histogram on actin bundles was fitted with the sum of 3 Gaussian components centered at −28.0 ± 18.8, 40.6 ± 11.6, and 72.7 ± 5.9 nm (n = 202). (D) Run-length of the chimera with the parallel coiled-coil domain at different ATP concentrations. Chimera with the parallel coiled-coil domain was nonprocessive at high ATP concentrations. (E) Velocity of the chimera at different ATP concentrations.