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Mutations in the antiparallel coiled-coil domain disrupt the dimerization of myosin X. (A) Schematic of mutant myosin X which contains four mutations in the antiparallel coiled-coil domain. (B) Representative fluorescent intensity trace of mutant myosin X showing one-step photobleaching. (C) Prevalence of one-step and two-step photobleaching of mutant myosin X (N = 40 molecules).
