Table 2. Run-Length and Velocity of Chimera with the Motor and Lever Arm Domain from Myosin X and the Parallel Coiled-Coil Domain from Myosin V on Actin Filaments and Actin Bundles at Different ATP Concentration.
Run-length
(μm) |
Velocity (nm/s) |
||||||||
---|---|---|---|---|---|---|---|---|---|
Actin track | ATP concentration | Mean | SD | SE | N | Mean | SD | SE | N |
Actin filaments | 2 μM | 0.43 | 0.13 | 0.03 | 23 | 57.2 | 24.9 | 5.2 | 23 |
20 μM | 0.67 | 0.37 | 0.08 | 20 | 288.7 | 138.1 | 30.9 | 20 | |
30 μM | 0.71 | 0.31 | 0.07 | 21 | 307.4 | 133.3 | 29.1 | 21 | |
2 mM | No processive motility | No processive motility | |||||||
Actin bundles | 2 μM | 0.51 | 0.24 | 0.05 | 24 | 63.9 | 23.8 | 4.9 | 24 |
20 μM | 0.67 | 0.42 | 0.09 | 22 | 174.2 | 68.0 | 14.5 | 22 | |
30 μM | No processive motility | No processive motility | |||||||
2 mM | No processive motility | No processive motility |