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. 2021 Feb 28;7(1):1–7. doi: 10.52601/bpr.2021.200047

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© The Author(s) 2021

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Lysosome acidity assay. A–C Confocal fluorescence images of the hypodermis at adult day 2 in wild type (WT), daf-2(e1370) and cup-5(bp510) expressing NUC-1::pHTomato controlled by the heat-shock (hs) promoter. Scale bars: 5 μm. D The average intensity of pHTomato per lysosome is quantified. At least 20 animals were scored in each strain. Data are shown as mean ± SD. The paired t-test was performed to compare the mutant datasets with the wild type. **p < 0.001