Figure 1.

Attachment of dermal cells to the epidermal cysts in skin organoid culture and hair regeneration upon transplantation. A. Schematic of an epithelial cyst. B. Collagen I immunostaining and wholemount scanning of skin organoid cultures of K14H2BGFP transgenic mice cells show the layers of dermal cells surrounding the epidermal cyst from D1 to D3. Scale bars, 50 um. C. Statistical of the dermal cells (left) and dermal layers (right) surrounding the epidermal cyst. D. Schematic of increased dermal cell attachment in skin organoid from D1 to D3. E. Time-lapse live imaging of Pdgfra-EGFP mouse cells shows dermal cell initial attachment during 9-22 h. Schematic of dermal cell initial attachment and movement during 9-22 h. Dark and light blue represent active and inactive cells, respectively. The analysis was based on the movies published in our previous study ¹. F. Statistical of the active or inactive number of dermal cells during 9-22 h and 53-64 h. G. Time-lapse live imaging of Pdgfra-EGFP mouse cells shows dermal cells move around the epidermal cyst during 53-64 h. Schematic of dermal cell movement during 53-64 h. The analysis was based on the movies published in our previous study ¹. H. Statistical of the active or inactive number of dermal cells in the 1-3 layers of the dermal layers during 9-22 h and 53-64 h. N ≥ 3, **p < 0.01, *p < 0.05, and # no significant change. I. Schematic of hair regeneration from D4 skin organoid (left panels 1 and 2); K14 immunostaining and H&E staining show the cyst structure formation in the hair regeneration (mid-panels 1 and 2); hair regeneration from the skin cysts, and statistical of the number of regenerated hair follicles (right panels 1 and 2).