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. 2023 May 8;13(9):2863–2878. doi: 10.7150/thno.81240

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Genetic or pharmacological inhibition of NOX4 attenuated ROS production and NF-κB p65 signaling activation in injured kidneys. (A) ROS were assessed in situ by DHE staining (×200, scale bars = 50 μm). (B) The protein expression levels of p-IκBα, IκBα, p-p65 and p65 in the kidneys were analyzed by western blot analysis and quantified by densitometry. Data are represented as the mean ± SD, n = 5. ^****P < 0.0001 for NOX4^fl/fl/LPS vs NOX4^fl/fl/Saline or for LPS vs Ctrl; ^####P < 0.0001 for NOX4^tecKO/LPS vs NOX4^fl/fl/LPS or for LPS + GKT vs LPS.