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. 2023 May 8;13(9):2757–2773. doi: 10.7150/thno.81406

Figure 8.

Figure 8

Upregulation of STAT1/HMGB1 in kidney tissues of CKD patients. The dataset GSE66494 deposited in GEO for kidney tissues from CKD patients and healthy donors was analyzed for gene transcription (A-C). Renal biopsies from non-CKD patient controls (n=3) and CKD patients (n=7) were assessed for the immunofluorescence staining (D-G). (A) Upregulation of HMGB1 in kidney tissues of CKD patients. Data are expressed as mean ± SEM. N: 8 healthy subjects and 54 CKD samples. * P <0.05 vs. the control group (Con). (B) Bubble graphs of the enriched gene expression pathways based on GO enrichment analysis. (C) Heat-map of differentially expressed genes (DEGs) belonging to the STAT/HMGB1 signaling pathway kidney tissues of CKD patients. (D) Representative immunofluorescence staining of HMGB1in renal biopsies from CKD patients. (E) Statistical analysis of the average fluorescence intensity of HMGB1. (F) Representative immunofluorescence staining of p65. (G) Statistical analysis of number of p65-positive cell nuclei in the area of the field. Scale bar = 50 μm. Quantitative data are expressed as mean ± SEM. N ≥ 3. * P <0.05 vs. the non-CKD group.