Table 1. TPS homologs identified in this study.
| Name | Contig ID | CDS (bp) | Protein (aa) | Predicted localization3) | BlastX top hit against uniprot database4) | Predicted TPS cluster based on phylogenetics | Major products identified in in vivo enzyme assays |
|---|---|---|---|---|---|---|---|
| AaTPS1 | comp121029 | 1,854 | 617 | Other | Isoprene synthase from Populus alba (Q50L36, 0.0) | hemiTPS | No product |
| AaTPS2 | comp128785 | 1,785 | 594 | Chloroplast (42) | (−)-α-Terpineol synthase from Vitis vinifera (Q6PWU2, 0.0) | monoTPS | α-Phellandrene and β-phellandrene |
| AaTPS3 | comp129373 | 1,770 | 589 | Other | (+)-Limonene synthase from Citrus sinensis (A0A1C9J6A7, 0.0) | monoTPS | α-Pinene |
| AaTPS4 | comp115931 | 1,758 | 585 | Chloroplast (29) | (+)-α-Terpineol synthase from Vitis vinifera (F6M8I0, 0.0) | monoTPS | α-Pinene |
| AaTPS5 | comp128661 1) | 1,6502) | 5492) | Other | (R)-Limonene synthase 1 from Vitis vinifera (A0A1C9J6A7, 0.0) | monoTPS | β-Bisabolene |
| AaTPS6 | comp136494 | 1,713 | 570 | Other | α-Copaene synthase from Eleutherococcus trifoliatus (Η9L9Ε5, 0.0) | sesquiTPS | β-Cedrene, β-barbatene, and β-farnesene |
| AaTPS7 | comp120360 | 1,692 | 563 | Other | α-Farnesene synthase from Ricinus communis (Β9RXW0, 3e-145) | sesquiTPS | α-Bergamotene |
| AaTPS8 | comp129101 | 1,701 | 566 | Other | Kunzeaol synthase from Thapsia garganica (K4LMW2, 0.0) | sesquiTPS | α-Humulene and germacrene D |
| AaTPS9 | comp127914 | 1,641 | 546 | Other | Valerianol synthase from Camellia sinensis (A0A167V661, 5e-167) | sesquiTPS | δ-Cadinene |
| AaTPS10 | comp1232041) | 1,551 | 516 | Other | (3S,6E)-Nerolidol synthase 1 from Fragaria×ananassa (P0CV94, 0.0) | sesquiTPS | β-Farnesene |
| AaTPS11 | comp1193611) | 2,3252) | 7742) | Other | ent-Kaurene synthase from Vitex agnus-castus (A0A2K9RFY0, 0.0) | diTPS | No product |
1)Contigs including a partial CDS. 2)The sequences for comp119361 and comp128661 were deduced from the full-length cDNA sequences amplified by RT-PCR after determination of the terminal sequences by means of 5′- and 3′-RACE, respectively. 3)Predicted by ChloroP, Figures in parenthesis show length (in amino acids) of predicted transit peptide. 4)Top hit results with accession number and e-value in parentheses.