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. 2022 Dec 25;39(4):421–425. doi: 10.5511/plantbiotechnology.22.1031a

Figure 2. Expression patterns of SlRMA1 and SlRMA2 in tomato. Relative transcript levels were measured by RT-qPCR. The primers used are listed in Supplementary Table S1. Average values and standard deviations of three biological replicates are shown. (A) Expression in the wild type and jre4-1. The leaves from 5-week-old plants were used for analysis. Expression levels in wild-type tissue were set to 1. Significant differences relative to the controls were assessed by Student’s t-test. * p<0.05; ** p<0.01. (B) Gene expression in leaves treated with methyl jasmonate (MeJA), 1-aminocyclopropane-1-carboxylic acid (ACC), or both. Detached leaves from 5-week-old tomato plants were submerged for 24 h in solutions containing either or both chemicals, provided at 100 µM. Expression levels in the mock-treated controls were set to 1. Different lowercase letters indicate significant differences between values at p<0.05, as determined by one-way analysis of variance (ANOVA) followed by a Tukey–Kramer test. (C) Expression in various tissues of wild-type tomato. Open flowers, leaves, roots, and fruits at different ripening stages were examined. While values of flowers, leaves, and roots are shown in white bars, black and light grey bars represent data of green fruits and fruits after breaker stage, respectively. Expression levels in tissues with the highest expression were set to 1. Different lowercase letters indicate significant differences between values at p<0.05, as determined by one-way ANOVA followed by a Tukey–Kramer test.

Figure 2. Expression patterns of SlRMA1 and SlRMA2 in tomato. Relative transcript levels were measured by RT-qPCR. The primers used are listed in Supplementary Table S1. Average values and standard deviations of three biological replicates are shown. (A) Expression in the wild type and jre4-1. The leaves from 5-week-old plants were used for analysis. Expression levels in wild-type tissue were set to 1. Significant differences relative to the controls were assessed by Student’s t-test. * p<0.05; ** p<0.01. (B) Gene expression in leaves treated with methyl jasmonate (MeJA), 1-aminocyclopropane-1-carboxylic acid (ACC), or both. Detached leaves from 5-week-old tomato plants were submerged for 24 h in solutions containing either or both chemicals, provided at 100 µM. Expression levels in the mock-treated controls were set to 1. Different lowercase letters indicate significant differences between values at p<0.05, as determined by one-way analysis of variance (ANOVA) followed by a Tukey–Kramer test. (C) Expression in various tissues of wild-type tomato. Open flowers, leaves, roots, and fruits at different ripening stages were examined. While values of flowers, leaves, and roots are shown in white bars, black and light grey bars represent data of green fruits and fruits after breaker stage, respectively. Expression levels in tissues with the highest expression were set to 1. Different lowercase letters indicate significant differences between values at p<0.05, as determined by one-way ANOVA followed by a Tukey–Kramer test.