FIGURE 4.

An FRD modulates immune populations in the ileal lamina propria.

Mice were fed an FRD or ND for 4 or 12 wk before ileal lamina propria cells were isolated and analyzed by flow cytometry. tSNE analysis was performed on CD45⁺ cells previously gated on lymphocytes and single cells. (A) Schematic of the experimental procedure. (B) tSNE and FlowSOM analysis of flow cytometry data. (C) Table of selected markers expressed by the indicated populations taken from the tSNE analysis. (D) The number of cells in the indicated populations in each diet group. (E) Two-dimensional plot of Helios and RORγt in populations 3 and 4 from mice fed an FRD. n = 5 per group, two-way ANOVA for bar graphs. *p < 0.05.