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. 2023 Mar 20;7(3):213–227. doi: 10.4049/immunohorizons.2300008

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FIGURE 6. — An FRD can moderately modulate immune populations in the spleen over time.

Four- to 6-wk-old mice were fed an FRD for 4 or 12 wk before splenic immune cells were isolated and analyzed by flow cytometry. (A) Schematic of the experiment. tSNE analysis was performed on CD45⁺ cells previously gated on lymphocytes and single cells. (B) tSNE and FlowSOM analysis of splenic immune populations in each diet group. (C) Number of cells in selected populations in each diet group. (D) Histograms from flow cytometry depicting prominent identifying markers of the selected populations. n = 5 per group, two-way ANOVA for bar graphs.