Figure 4.

7-KC or H₂O₂ caused MIN6 cell senescence through the SIRT1–CDK4–Rb – E2F1 signaling pathway. (A – H) MIN6 cells were treated with 7-KC or H₂O₂ for 24 h, then SIRT1 (B), CDK4 (C), KMTb/EZH2 (D), E2F1 (E), p-Rb (F), γ-H2A.X (G), and p-p38MAPK (H) expression was detected by western blotting (A). *P < 0.05, **P < 0.01 vs. Normal control.

The protein expression showed that 7-KC or H₂O₂ caused MIN6 cell senescence through the inhibiting SIRT1 and CDK4–Rb – E2F1 signaling pathway. (A – H) MIN6 cells were treated with 7-KC or H₂O₂ for 24 h, then SIRT1 (B), CDK4 (C), KMTb/EZH2 (D), E2F1 (E), p-Rb (F), γ-H2A.X (G), and p-p38MAPK (H) expression was detected by western blotting (A). Normal control (2-hydroxypropyl-β-cyclodextrin solution); 7-KC (25 μmol/L 7-KC in 2-hydroxypropyl-β-cyclodextrin solution); H₂O₂(150 μmol/L H₂O₂ in 2-hydroxypropyl-β-cyclodextrin solution). *P < 0.05, **P < 0.01 vs. Normal control.