Table 1. Gyration radii, forward scattering intensities, molecular weights calculated from forward scattering intensities, and maximum pair distances for BSA20 and AF21.

R _g and i(0): gyration radius and concentration-normalized forward scattering intensity for non-treated SAXS, respectively. R _g1 and i ₁(0): gyration radius and concentration-normalized forward scattering intensity of the monomer, respectively, which were derived using AUC–SAS. M: molecular weight calculated from the forward scattering intensity. D _max: maximum pair distance from P ₁(r). The error of the gyration radius is the standard deviation. The errors of the concentration-normalized forward scattering intensity and molecular weight were calculated from the standard deviations of the forward scattering intensity and concentration.

	R g, R g1 (Å)	i(0), i 1(0) (mg−1cm2)	M (kDa)	D max
BSA20
Non-treated SAXS	30.9 ± 0.3	0.0565 ± 0.0005	84.2 ± 0.7	98.5
First AUC–SAS	28.1 ± 0.2	0.0461 ± 0.0004	68.7 ± 0.6	78.8
Improved AUC–SAS	27.3 ± 0.2	0.0468 ± 0.0004	69.6 ± 0.6	77.9
Crystal structure†	27.1	0.0465	69.2	77.0
AF21
Non-treated SAXS	61.5 ± 0.8	0.280 ± 0.010	604 ± 20	175
First AUC–SAS	55.1 ± 0.5	0.235 ± 0.009	507 ± 19	122
Improved AUC–SAS	54.1 ± 0.5	0.239 ± 0.009	516 ± 19	120
Crystal structure‡	54.0	0.221	477	119

^†PDB code 4f5s.

^‡PDB code 4v1w; Russo & Passmore (2014 ▸).