Table 2. Gyration radii and forward scattering intensities given by AUC–SAXS (improved AUC–SAXS) and SEC–SAXS for various proteins.
R g1 and i 1(0): gyration radius and concentration-normalized forward scattering intensity, respectively. The error of the gyration radius is the standard deviation. The error of the concentration-normalized forward scattering intensity was calculated from the standard deviations of the forward scattering intensity and concentration.
| Protein | Method | Sample code | R g1 (Å) | i 1(0) (mg−1cm2) |
|---|---|---|---|---|
| BSA | Improved AUC–SAXS | BSA6 (r a = 0.06) | 27.2 ± 0.2 | 0.0461 ± 0.0006 |
| BSA13 (r a = 0.13) | 27.3 ± 0.2 | 0.0460 ± 0.0005 | ||
| BSA20 (r a = 0.20) | 27.3 ± 0.2 | 0.0468 ± 0.0004 | ||
| SEC–SAXS | – | 27.2 ± 0.3 | 0.0455 ± 0.0019 | |
| AF | Improved AUC–SAXS | AF5 (r a = 0.05) | 54.4 ± 0.6 | 0.249 ± 0.008 |
| AF15 (r a = 0.15) | 54.3 ± 0.5 | 0.243 ± 0.009 | ||
| AF21 (r a = 0.21) | 54.1 ± 0.5 | 0.239 ± 0.009 | ||
| SEC–SAXS | – | 53.9 ± 0.6 | 0.241 ± 0.015 | |
| Catalase | Improved AUC–SAXS | Cat3 (r a = 0.03) | 37.2 ± 0.2 | 0.155 ± 0.006 |
| Cat8 (r a = 0.08) | 37.3 ± 0.2 | 0.153 ± 0.005 | ||
| SEC–SAXS | Cat8 (r a = 0.08) | 37.1 ± 0.2 | 0.160 ± 0.008 | |
| Lyz | Improved AUC–SAXS | Lyz6 (r a = 0.06) | 15.0 ± 0.2 | 0.0125 ± 0.0008 |
| SEC–SAXS | – | 15.1 ± 0.1 | 0.0124 ± 0.0012 | |
| βB2-cry | Improved AUC–SAXS | βB2-cry11 (r a = 0.11) | 22.6 ± 0.2 | 0.0319 ± 0.0007 |
| SEC–SAXS | – | 22.7 ± 0.3 | 0.0325 ± 0.0013 | |
| OVA | Improved AUC–SAXS | OVA4 (r a = 0.04) | 23.9 ± 0.2 | 0.0346 ± 0.0005 |
| SEC–SAXS | – | 23.9 ± 0.4 | 0.0340 ± 0.0009 | |
| RNaseA | Improved AUC–SAXS | RNaseA8 (r a = 0.08) | 15.0 ± 0.2 | 0.0110 ± 0.0004 |
| SEC–SAXS | – | 14.8 ± 0.2 | 0.0116 ± 0.0003 |