Figure 4.

Image-based compound screen and phenotypic landscape

(A) Scheme of experimental outline, image processing, and analysis of the screen.

(B) Left: representative maximum intensity projection images of whole gastruloid phenotypes. Stainings: DAPI, Sox1, Brachyury, and E-cadherin, Scale bars, 100 μm. Right: UMAP plot color-coded by whole gastruloid class. Data points: individual gastruloids, n = 8,740. Heatmap: mean values of indicated features for each class, Z score normalized.

(C) UMAP plots and pie charts color-coded by whole gastruloid classes from indicated treatment regimens (left to right: 32–72 h, 48–72 h, and 72–96 h).