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. Author manuscript; available in PMC: 2023 Jun 5.
Published in final edited form as: J Am Chem Soc. 2021 Mar 29;143(13):4910–4914. doi: 10.1021/jacs.1c01045

Figure 3.

Figure 3.

Activation of TRAF6-N by viperin. Top: Representative Coomassie-stained gel showing consumption of ubiquitin and formation of ubiquitin oligomers, note the smear of high Mr species at longer times. Bottom: Quantification of mono-, di-, and triubiquitin; these oligomers are rapidly depleted as they are converted to higher Mr species. (Experiments were performed with 1:1 molar ratio of TRAF6-N to viperin.)