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. Author manuscript; available in PMC: 2023 Jun 5.
Published in final edited form as: Nat Protoc. 2022 Feb 23;17(4):941–961. doi: 10.1038/s41596-021-00657-4
Component Amount Final
pBait plasmid (40–80 ng/μL) 1 μL 40–80 ng
pPrey plasmid (40–80 ng/μL) 1 μL 40–80 ng
pAdapter plasmid (40–80 ng/μL) 1 μL 40–80 ng
heat-shock competent reporter-strain cells 30 μL
Total 33 μL