| Step | Temperature (°C) | Time |
|---|---|---|
| Initial denaturation | 95°C | 30 seconds |
| 40 cycles | 95°C | 30 seconds |
| 50–60°C (annealing) | 30 seconds | |
| 68°C | 1 minute per kb | |
| Final elongation | 68°C | 5 minutes |
| Hold | 10°C |
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