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. 2023 May 19;12:e86556. doi: 10.7554/eLife.86556

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© 2023, Williams, Ngo et al

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Figure 6. — (A) Airyscan 3D projection of U-2 OS cells expressing ANXA6(FL)-mNG with endogenous CD63 and wheat germ agglutinin (WGA) counterstain upon treatment with DMSO or 1 µM ionomycin for 10 min. Green: ANXA6(FL)-mNG; magenta: endogenous CD63 immunofluorescence; blue: WGA CF640R conjugate. Scale bars: 10 µm. (B) Airyscan 3D projection of U-2 OS cells expressing ANXA6(N)-mNG and ANXA6(C)-mSc with WGA counterstain upon treatment with DMSO or 1 µM ionomycin for 10 min. Green: ANXA6(N)-mNG; magenta: ANXA6(C)-mSc; blue: WGA CF640R conjugate. Scale bars: 10 µm. (C) Airyscan 3D projection of U-2 OS cells expressing ANXA6(N)-mNG and ANXA6(C)-mSc with endogenous CD63 upon treatment with DMSO or 1 µM ionomycin for 10 min. Green: ANXA6(N)-mNG; magenta: ANXA6(C)-mSc; blue: endogenous CD63 immunofluorescence. Scale bars: 10 µm.