Table 1.
Primers and probe for RT-qPCR.
| Primer name | Accession number/GenBank | Forward primer 5′-Sequence-3′ |
Reverse primer 5′-Sequence-3′ |
Probe |
|---|---|---|---|---|
| DTMUV Envelope Gene | MH460536.1 | TGTCTTATGCAGGTACCGATG | CGTATGGGTTGACTGTTATCA | FAM-AGTTCCCATATCCATGTC -MGB |
| 34 kDa salivary protein | AALF004421 | TCAAAAGCGCCATCTACAGT | CTGGGTCTTCAATACCTCCA | |
| MCR | AALF005612 | TCCGCCCCGGTCAGGTTTACAA | TCGACGCCATCCCGGGAGATAC | |
| Ribosomal Protein S7 | AALF016123 | GCGCGTCAAGCTGGATGGATCGCA | TCCGGTCAGCTTCTTGTACACCGA | |
| CECA | AALF000656 | GCTGGGTCAAACCGAAGCCGG | ATTTTCCAAGTGCCTTGATTCC | |
| CECB | AALF014650 | ATGAACTTCAACAAACTGTTCC | AACACACGCTTGCCGGCCTTT | |
| CECD | AALF043934 | ACTTCAGCAAACTGTTTGCGCTTG | CCTTGAACACTCGCTTGCCGA | |
| CECE | AALF024715 | GCTCTGCTTTTCACTGCCCAAACC | TGCAGCCCCAGAACAGTTGGGA | |
| CECG | AALF026733 | CTGGCGGACGAGGTTCGTTCTTT | ACCCCGAATCCACTCAGCAGATCG | |
| CECI | AALF012131 | AAGAAGCTGTTCATCTTCGTC | TCACTTTGTCGACAGCATGGGC | |
| DEFA | AALF065006 | AACTTGCGCCTCAAGCGGGCCACC | TCAGTTCCGGCAGACGCACACCTT | |
| DEFC | AALF008821 | GAGCTGTCCGACGATGTCCGCTC | GCACTATCCCCAACACCGAA | |
| DEFD | AALF003252 | CTCGTTCAAGCGATATCAGTTC | TCGTCCGCCAGCACCGATTC | |
| DEFE | AALF008822 | GAATTGCTACGCCGAAAGCGC | ACAAACGCAGACTCCCCGATC | |
| ATT | AALF048664 | GAATCCCTAGCTCAGTTTCA | AAACTGGTCCTCCCTTGGTGTT | |
| DPT | AALF044217 | TTCGGGGCTACGCACCAGCAGGA | CGTCGCCCTGAAATCCACCGAA | |
| GAM | AALF001757 | CGAGAGCCAAAACCTGTGCC | TGCAGCTGGCTATCGAATTCGC |