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. 2023 Jun 5;13:9102. doi: 10.1038/s41598-023-35541-6

Figure 2.

Figure 2

Protein tyrosine phosphorylation. Three major fluorescent patterns viz. non-fluorescent spermatozoa i.e. non-phosphorylated (NP), sperm bearing signal at equatorial region and mid-piece (EM) and spermatozoa with a signal at the acrosomal region, equatorial region and mid-piece (AEM).were observed during immunocytochemistry using a monoclonal anti-phosphotyrosine antibody (P1869; Sigma). The buffalo spermatozoa were exposed to 0.5, 1, and 2 μM of OPPMs viz. Omethoate (A), Paraoxon methyl (B), and TCPy (C) for 2 h.