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. 2023 May 5;26(6):106817. doi: 10.1016/j.isci.2023.106817

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© 2023 The Author(s)

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

PMC Copyright notice

Phf6^R274X mutation alters the gene expression profiles in HSC/HPCs

(A) Gene interaction analysis showing the significantly altered expression pattern in Vav1-Cre;Phf6^fl-R274X/Y BM LSK⁺ cells compared with control cells.

(B) Heatmap showing differential expression of 66 genes related to leukocyte proliferation and differentiation in Phf6^fl-R274X/Y and Vav1-Cre;Phf6^fl-R274X/Y mice BM LSK⁺ cells. |Log2foldchange | > 1, p value <0.05 (n = 3).

(C) Gene set enrichment analysis (GSEA) for genes affected in the BM LSK⁺ cell from Phf6^fl-R274X/Y and Vav1-Cre;Phf6^fl-R274X/Y mice.

(D and E) GO and KEGG analysis of the up-regulated genes in Vav1-Cre;Phf6^fl-R274X/Y cells compared with Phf6^fl-R274X/Y cells.

(F and G) Relative expression levels of Aif1, Cd3e, Igf1, and Xbp1 genes in BM LSK⁺ cells from Phf6^fl-R274X/Y and Vav1-Cre;Phf6^fl-R274X/Y mice. mRNA levels were normalized to the expression of GAPDH. Data information: in (F-G) data are shown as the mean ± SD. ∗p < 0.05, ∗∗p < 0.01, and ∗∗∗p < 0.001 by the Student’s t test.