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. 2023 May 23;13:1192134. doi: 10.3389/fcimb.2023.1192134

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Copyright © 2023 Jia, Wang, Liu, Huang, Xiao, Fu, Sun, Xu, Wang, Zhou and Wang

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Performance of the MTB-MCDA-CRISPR detection system in clinical samples. A total of 96 samples were tested by the MTB-MCDA-CRISPR assay to validate the clinical feasibility. The generated fluorescence signals were recorded by the real-time PCR platform (A) and visually interpreted by naked eye under blue light (B). Signals/tubes 1-19 present the results of the 19 samples from non-TB patients, and Signals/tubes 20-96 represent the results of the 77 samples from TB patients.