Table 2.
Primer sequences used in the study.
| Primers | Sequence and modification (5’-3’) a | Lengthb/c |
|---|---|---|
| F1 | GCGGTCACAGATGTCCAG | 18 nt |
| F2 | CCTGCATCCCGACATCGT | 18 nt |
| CP1 | AGACCAGCGGCAACAGCGG-TT-CAGTTCTTGGGCCGACAC | 41 nt |
| CP2 | AAGTCTCGGTGGCCAAGACGCAACGCATCCCAACGGA | 37 nt |
| C1 | AGACCAGCGGCAACAGCGG | 19 nt |
| C2 | AAGTCTCGGTGGCCAAGACG | 20 nt |
| D1 | CTCAGTTGCCCTTCCCTA | 18 nt |
| D2 | GCGGCAGTGAACGTCAT | 17 nt |
| R1 | GTTCATCGTCACGGAAC | 17 nt |
| R2 | TCCGCCCACCGAAAA | 15 nt |
| gRNA | UAAUUUCUACUAAGUGUAGAUAGUUCUUGGGCCGACACGUA | 41 mer |
| Probe c | FAM-TATTATTATTATTATTT-BHQ1 | 17 mer |
a
CP1, primer were modified in the linker region with a PAM site (TT).
b
nt, nucleitide.
c
mer, monomeric unit.