Skip to main content
. 2023 May 23;4:1171188. doi: 10.3389/fpain.2023.1171188

Figure 3.

Figure 3

Total expression of ABHD6 and MAGL in PAG after cortical KCl injection. Female Sprague Dawley rats were injected with KCl (0.5 µl, 1M) or aCSF (0.5 µl) through the guide cannula one week after dural cannulation surgery. PAG samples obtained 30 and 90 min after cortical injections were subjected to Western immunoblotting to determine expression of ABHD6 and MAGL. (A) Schematic of experimental setting to detect changes in the expression of MAGL and ABHD6 in CSD induced model. (B) Representative immunoblots showing the detection of MAGL and ABHD6 along with total protein in PAG samples 30 min after cortical injection of KCl or aCSF. (C) Representative immunoblots of PAG samples showing the detection of MAGL and ABHD6 with total protein 90 min after CSD induction. (D) Cortical KCl significantly increased ABHD6 detection 30 min after injection however the detection of ABHD6 was significantly reduced at the 90 min time-point (unpaired t-test, 30min: t(5) = 3.44, p = 0.018; 90 min: t(4) = 3.752, p = 0.020) (E) MAGL detection was increased 30 min post-injection as compared to cortical injection of aCSF at times when 2-AG are reduced, but no significant difference was observed at 90 min in PAG samples (unpaired t-test, 30 min: t(6) = 3.762, p = 0.0094; 90 min: t(4) = 1.133, p = 0.32). Values are % of aCSF control ± SD (n = 3/condition) ns = non-significant (p > 0.05), *p < 0.05, **p < 0.01.