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. 2023 Jun 5;4(3):e293. doi: 10.1002/mco2.293

FIGURE 5.

FIGURE 5

LATS1/YAP1 regulated the polarization state of M1/M2 macrophage in BMMs. Cells were transfected with siRNA‐YAP1 (si‐Yap1), siRNA‐LATS1 (si‐Lats1), or siRNA negative control (si‐NC). 72 h after siRNA transfection, the cells were collected for qRT‐PCR and western blotting assays. (A) Western blotting and densitometric quantification of p‐YAP1, YAP1, LATS1, CCL2, CD80, iNOS, CD206, and Arg1. (B) Western blotting and densitometric quantification of p‐YAP1, YAP1 in the nucleus and cytoplasm. (C) The mRNA expression of Yap1, Ccl2, Cd80, Nos2, Mrc1, and Arg1 determined by qRT‐PCR. All data are presented as the mean ± SD (n = 5). ns, no significance; *p < 0.05, **p < 0.01, ***p < 0.001 between two groups. LATS1, large tumor suppressor 1 (gene symbol: Lats1).