TABLE 2.
Antibodies for flow cytometry.
| Panel | Antibody | Target population | Dilution |
|---|---|---|---|
| A & B | Anti‐CD45 (BD Biosciences, 557235; Clone 30‐F11) | Immune cells | 1:100 |
| A & B | Anti‐CD11b (eBio‐ sciences, 47‐0112‐82; Clone M1/70) | Myeloid cells | 1:200 |
| A | Anti‐NK1.1 (eBiosciences, 12‐5941‐82; Clone PK136) | NK cells | 1:50 |
| A | Anti‐ CD3 (eBiosciences, 17‐0031‐82; Clone 145‐2C11) | T‐cells | 1:62.5 |
| A | Anti‐CD4 (BioLegend, 100510; Clone RM4‐5) | CD4+ T cells | 1:75 |
| A | Anti‐CD8a (eBiosciences, 17‐0031‐82; Clone 145‐2C11) | CD8+ T cells | 1:250 |
| B | Anti‐F4/80 (BioRad, MCA497PE; Clone Cl:A3‐1) | Macrophages | 1:25 |
| B | Anti‐CD206 (biolegend, 141707; Clone C068C2) | M2‐like macrophages | 1:60 |
| B | Anti‐MHCII (BioLegend, 107616; Clone M5/114.15.2) | MHCII high macrophages | 1:250 |
| B | Anti‐CD19 (BioLegend, 115519; Clone 6D5) | B cells | 1:122 |