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. 2023 Jun 5;220(9):e20212276. doi: 10.1084/jem.20212276

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© 2023 Moriya et al.

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Figure 3. — NF-κB reporter assay. (A) 25 ng plasmids encoding WT or mutant RelA protein was used. The p.E473Rfs*18 and p.H487Tfs*7 RELA variants were hypomorphic, having ∼8.0 and 32.3% residual activity, respectively, whereas the other five mutants, including p.R246*, were classified as LOF. (B) The total amount of expression vector, containing WT (12.5 or 25 ng) and mutant (12.5, 25, or 37.5 ng) RELA, was adjusted to 50 ng by supplementation with EV. All of the mutants, except for p.R246* mutants, which cause RELA haploinsufficiency, showed a dose-dependent negative effect against WT RelA. The mean ± SEM of three independent experiments is shown.