Figure 2.

scRNA-seq analysis of TI characteristics

(A) Pathway enrichment analysis using up- and downregulated TIGs in high and low responders in monocytes separately. Pink area means pathways were upregulated, while the blue area means pathways were downregulated. Different patterns were shown between high and low responders. Specifically, IFN-γ pathways were upregulated in high responders but downregulated in low responders. See also Figures S2C–S2J.

(B) Effect size (log₂FC) comparison of TIGs identified in monocytes. Values on the x axis were log₂FC in high responders, while values on the y axis were from low responders. Yellow dots represent those TIGs only significant in high responders while dark-blue ones were only significant in low responders. Gray dots represent TIGs that were significant in both groups.

(C) Enriched pathways using TIGs that were significantly upregulated in high responders but downregulated in low responders. See also Figure S3A.

(D and E) Cell-cell interaction between monocytes and CD8⁺ T cells. Ligands were highly variable genes in senders, and targets were TIGs in receivers. In (D), monocytes were set as sender, while in (E) CD8⁺ T cells were set as sender. See also Figures S3B–S3E.