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. 2023 May 7;42(5):112487. doi: 10.1016/j.celrep.2023.112487

Figure 4.

Figure 4

TI characteristics of four monocyte subpopulations

(A and B) The number of up- and downregulated TIGs in each subpopulation. The results of pathway enrichment analysis using up- and downregulated TIGs identified in (A), separately, are summarized in (B). See also Figures S4C–S4F. Pathways in the pink area were upregulated and in the blue area downregulated. IFN-γ pathways were upregulated in TM1 but downregulated in TM2.

(C and D) Comparisons of the number of shared and unique TIGs in high and low responders in each population. The results of enrichment analysis (C) using specific up- and downregulated TIGs in high and low responders, separately, are summarized in (D). Pathways in the pink area were upregulated and in the blue area downregulated. Interestingly, IFN-γ pathways were upregulated in TM1 in high responders but were downregulated in TM2 in low responders. This again indicated the importance of IFN-γ pathways in TI and the role of these pathways in explaining individual diversity in responding to TI. See also Figures S5C–S5H and S6A–S6D.

(E) Further investigation comparing monocytes and CD8+ T cells explored communications between four monocyte subpopulations and CD8+ T cells. The expression level of the top 20 most active ligands identified in four subpopulations are shown as a dot plot. Different ligands were interacting with CD8+ T cells in each subpopulation, and TM1 was the most active cell type. Heatmap shows the potential regulatory relationship between ligands in monocyte subpopulations and TIGs in CD8+ T cells. See also Figures S6E–S6G.