REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
19.7E	Robinson et al., 2016	Patent WO2018106712A1
12.1F	Robinson et al., 2016	Patent WO2018106712A1
25.10C	Robinson et al., 2016	Patent WO2018106712A1
37.7H	Robinson et al., 2016	Patent WO2018106712A1
S370.7	This study	N/A
StrepMAB-Classic Oyster 645 conjugate	IBA Lifesciences	Cat# 2-1555-050
Cy3 conjugated goat-anti-human	Jackson Immuno Research	Cat# 109-165-008; RRID: AB_2337720
Totalseq-C Anti-Human Hashtag #1	Biolegend	Cat# 394661; RRID: AB_2801031
Totalseq-C Anti-Human Hashtag #2	Biolegend	Cat# 394663; RRID: AB_2801032
Totalseq-C Anti-Human Hashtag #3	Biolegend	Cat# 394665; RRID: 2801033
Totalseq-C Anti-Human Hashtag #4	Biolegend	Cat# 394667; RRID: 2801034
APC-Cy7 Mouse Anti-Human CD3	BD Biosciences	Cat# 557757; RRID: AB_396863
APC-Cy7 Mouse Anti-Human CD4	Biolegend	Cat# 317418; RRID: AB_571947
APC-Cy7 Mouse Anti-Human CD8	BD Biosciences	Cat# 557760; RRID: AB_396865
APC-H7 Mouse Anti-Human CD14	BD Biosciences	Cat# 561384; RRID: AB_10611720
PerCP-Cy5.5 Mouse Anti-Human CD19	Biolegend	Cat# 302230; RRID: AB_2073119
Bacterial and Virus Strains
NEB 5-alpha Competent E. coli (High Efficiency)	New England Biolabs	Cat# C2987H
Lassa virus (Josiah strain)	Brouwer et al., 2022	N/A
Biological Samples
Convalescent patient serum	Robinson et al., 2016	N/A
Chemicals, Peptides, and Recombinant Proteins
Tween20	Sigma-Aldrich	Cat# P1379-500ML
Bovine Serum Albumin (fraction V)	Thermo Scientific	Cat# 9048-46-8
Penicillin	Sigma-Aldrich	Cat# P3032-10MI
Streptomycin	VWR	Cat# 382-EU-100G
Iodacetamide	Sigma-Aldrich	Cat# I1149
BioLock solution	IBA Lifesciences	Cat# 2-0205-050
PBS	Thermo Scientific	Cat# 10010023
TBS	Alfa Aesar	Cat# J60764.K2
PEI MAX	Polysciences	Cat# 24765-1
Uranyl Formate	Electron Microscopy Sciences	Cat #D310 25 GM
Fluorinated octyl maltoside	Anatrace	Part# O310F
Imidazole	Sigma-Aldrich	Cat# I5513
Papain from papaya latex	Sigma-Aldrich	SKU# P4762
Acetonitrile, 80%, 20% Water with 0.1% Formic Acid, Optima LC/MS	Fisher Scientific	Cat# 15431423
Water with 0.1% Formic Acid (v/v), Optima™ LC/MS Grade	Fisher Scientific	Cat# LS118-212
Acetonitrile	Fisher Scientific	Cat# 10489553
Trifluoroacetic acid	Fisher Scientific	Cat# 10155347
Dithiothreitol	Sigma-Aldrich	Cat# 43819
Mass spectrometry grade trypsin	Promega	Cat# V5280
Sequencing grade chymotrypsin	Promega	Cat# V1061
α-Lytic Protease	New England Biolabs	Cat# P8113L
Urea	Sigma-Aldrich	U5378-1KG
Fugene	Promega	Cat# E5911
DEAE-dextran	Sigma-Aldrich	Cat# D9885
Saquinavir	NIH-ARP	Cat# 4658
Reporter Lysis buffer	Promega	Cat# E3971
Fetal Bovine Serum	Omega Scientific, Inc.	FB-02
RPMI 1640	Corning	15-040-CV
Trypan Blue	Sigma-Aldrich	T8154-100ML
Ethanol	Pharmaco	111000200
FectoPRO	Polyplus Transfection	101000019
Citric acid	Fisher Scientific	BP339-500
Tris Base	Sigma-Aldrich	T1503-5KG
Critical Commercial Assays
Bright-Glo Luciferase Assay System	Promega	Cat# E2620
Deposited Data
3D map of LIV GPC-I53-50A (strain Josiah)	This study	EMDB: EMD-28178
3D model of LIV GPC-I53-50A (strain Josiah)	This study	PDB: 8EJD
3D map of LII GPC-I53-50A (strain NIG08-A41)	This study	EMDB: EMD-28179
3D model of LII GPC-I53-50A (strain NIG08-A41)	This study	PDB: 8EJE
3D map of LV GPC-I53-50A (strain Soromba-R)	This study	EMDB: EMD-28180
3D model of LV GPC-I53-50A (strain Soromba-R)	This study	PDB: 8EJF
3D map of LVII GPC-I53-50A (strain Soromba-R)	This study	EMDB: EMD-28181
3D model of LVII GPC-I53-50A (strain Soromba-R)	This study	PDB: 8EJG
3D map of LIV GPC-I53-50A (strain Josiah_ bound to 12.1F Fab	This study	EMDB: EMD-28182
3D model of LIV GPC-I53-50A (strain Josiah_ bound to 12.1F Fab	This study	PDB: 8EJH
3D map of LIV GPC-I53-50A (strain Josiah_ bound to 19.7E Fab	This study	EMDB: EMD-28183
3D model of LIV GPC-I53-50A (strain Josiah_ bound to 19.7E Fab	This study	PDB: 8EJI
3D map of LIV GPC-I53-50A (strain Josiah_ bound to S370.7 Fab	This study	EMDB: EMD-28184
3D model of LIV GPC-I53-50A (strain Josiah_ bound to S370.7 Fab	This study	PDB: 8EJJ
Mass spectrometry data	This study	ftp://massive.ucsd.edu/MSV000091003/
S370.7 HC sequence	This study	GenBank: OQ451467
S370.7 LC sequence	This study	GenBank: OQ451468
Experimental Models: Cell Lines
FreeStyle 293F cells	Thermo Scientific	Cat# R79007
HEK 293T cells	ATCC	Cat# CRL-11268
Expi293T cells	ATCC	CRL-3216
TZM-bl cells	NIH ARRRP	Cat# 8129
Experimental Models: Organisms/Strains
Recombinant DNA
GPCysR4(Josiah)-StreptagII pPPI4 plasmid	Brouwer et al., 2022	N/A
GPCysR4(Josiah)-I53-50A.1NT1-Strep-tagII pPPI4 plasmid	Brouwer et al., 2022	N/A
GPCysR4(NIG08-A41)-I53-50A.1NT1-Strep-tagII pPPI4 plasmid	This study	N/A
GPCysR4(Soromba-R)-I53-50A.1NT1-Strep-tagII pPPI4 plasmid	This study	N/A
GPCysR4(Togo)-I53-50A.1NT1-Strep-tagII pPPI4 plasmid	This study	N/A
GPCysR4(Josiah)-I53-50A.1NT1-Avi-His pPPI4 plasmid	Brouwer et al., 2022	N/A
GPCysR4(NIG08-A41)-I53-50A.1NT1-Avi-His pPPI4 plasmid	Brouwer et al., 2022	N/A
GPCysR4(Soromba-R)-I53-50A.1NT1-Avi-His pPPI4 plasmid	This study	N/A
GPCysR4(Togo)-I53-50A.1NT1-Avi-His pPPI4 plasmid	This study	N/A
GPCysR4(Soromba-R_D114N)-I53-50A.1NT1-StrepTagII pPPI4 plasmid	This study	N/A
Furin pPPI4 plasmid	Brouwer et al., 2019	N/A
S1P pcDNA3.0 plasmid	This study	N/A
GPCysRRLL(Josiah)-I53-50A.1NT1-Strep-tagII pPPI4 plasmid	This study	N/A
Fc-tagged LAMP-1 ectodomain	Jae et al., 2014	N/A
GPC(NIG08-A41)_full-length pPPI4 plasmid	Brouwer et al., 2022	N/A
GPC(Josiah)_full-length pPPI4 plasmid	Brouwer et al., 2022	N/A
GPC(CSF)_full-length pPPI4 plasmid	Brouwer et al., 2022	N/A
19.7E HC, 19.7E LC, 12.1F HC, 12.1F LC, 25.10C HC, 25.10C LC, 37.7H HC, 37.7H LC gene fragments	Integrated DNA Technologies	N/A
S370.7 HC and LC gene fragments	This study	N/A
Software and Algorithms
GraphPad Prism v8	GraphPad	N/A
UCSF Chimera	Pettersen et al., 2004	N/A
UCSF ChimeraX	Goddard et al., 2018	N/A
cryoSPARC.v3	Punjani et al., 2017	N/A
Leginon	Suloway et al., 2005	N/A
MotionCor2	Zheng et al., 2017	N/A
GCTF	Zhang, 2016	N/A
Relion/3.0 and 3.1	Zivanov et al., 2018	N/A
ABodyBuilder	Leem et al., 2016	N/A
Coot	Casañal et al., 2020	N/A
Phenix	Liebschner, et al., 2019	N/A
EMRinger	Barad et al., 2015	N/A
MolProbity	Williams et al., 2018	N/A
Appion	Lander et al., 2009	N/A
Epitope-Analyzer	Montiel-Garcia, et al., 2022	N/A
PDBePISA	Krissinel et al., 2007	N/A
Privateer	Agirre et al., 2015	N/A
Byos™ (Version 4.0)	Protein Metrics Inc.	N/A
XCalibur Version v4.2	Thermo Fisher	N/A
Orbitrap Fusion Tune application v3.1	Thermo Fisher	N/A
Cellranger 6.0.2	10X Genomics	N/A
ab[x] toolkit	Briney et al., 2018	N/A
Single Cell Analysis of B cells (SCAB)	Hurtado et al., 2022	N/A
GenePix Pro 7 software v7.2.29.2	Molecular Devices	N/A
Other
PstI-HF	New England Biolabs	Cat# R3140S
BamHI-HF	New England Biolabs	Cat# R3136S
NotI-HF	New England Biolabs	Cat# R3189
Q5 Site-directed mutagenesis kit	New England Biolabs	Cat# E0554S
T4 DNA ligase	New England Biolabs	Cat# M0202
Quick ligation kit	New England Biolabs	Cat# M2200
Superdex200 10/300GL Column	GE Healthcare Life Sciences	Cat# 28990944
Protein G resin	Cytiva	Cat# 17061802
CaptureSelect IgG-Fc resin	Thermo Scientific	Cat# 2942852010
Ni-NTA agarose	QIAGEN	Cat# 30210
Amicon® Ultra-4 Centrifugal Filter Unit (100 kDA MWCO)	Millipore Sigma	SKU# UFC810024
Amicon® Ultra-4 Centrifugal Filter Unit (30 kDA MWCO)	Millipore Sigma	SKU# UFC803024
Amicon® Ultra-4 Centrifugal Filter Unit (10 kDA MWCO)	Millipore Sigma	SKU# UFC801024
Amicon Ultra-0.5 Centrifugal Filter Unit (100 kDa MWCO)	Millipore Sigma	SKU# UFC5100BK
Amicon Ultra-0.5 Centrifugal Filter Unit (30 kDa MWCO)	Millipore Sigma	SKU # UFC5030BK
Amicon Ultra-0.5 Centrifugal Filter Unit (10 kDA MWCO)	Millipore Sigma	SKU# UFC5010BK
Prometheus NT.Plex nanoDSF Grade High Sensitivity Capillary Chips	Nanotemper	Cat# PR-AC006
400-mesh copper grids	Electron Microscopy Sciences	Cat# 0400-Cu
Octet Red96 system	Sartorius (FortéBio)	N/A
Octet Biosensors: Streptavidin (SA)	Sartorius (FortéBio)	Cat# 18-5019
Octet Biosensors: Anti-Human Fc Capture (AHC)	Sartorius (FortéBio)	Cat# 18-5060
FreeStyle 293 Expression medium	Thermo Scientific	Cat# 12338018
OptiMEM	Gibco	Cat# 31985-070
DMEM	Gibco	Cat# 21969-035
Fetal calf serum	Gibco	Cat# 10270/106
BioLock	IBA Lifesciences	Cat# 2-0205-250
BXT Buffer (10X)	IBA Lifesciences	Cat# 2-1042-025
Steritop Filter Units	Merck Millipore	Cat# C3239
Greiner CELLSTAR® 96 well plates round bottom clear wells	Merck Millipore	Cat# M9436
Totalseq-C0951 PE-Streptavidin	Biolegend	Cat# 405183
Totalseq-C0992 PE-Streptavidin	Biolegend	Cat# 405181
Totalseq-C0994 PE-Streptavidin	Biolegend	Cat# 405177
Totalseq-C0956 APC-Streptavidin	Biolegend	Cat# 405261
Totalseq-C0958 APC-Streptavidin	Biolegend	Cat# 405293
Totalseq-C0971 Streptavidin	Biolegend	Cat# 405271
Totalseq-C0972 Streptavidin	Biolegend	Cat# 405273
Totalseq-C0973 Streptavidin	Biolegend	Cat# 405275
LIVE/DEAD Fixable Aqua Dead Cell Stain Kit	Thermo Scientific	Cat# L34966
Biotinylated Human Serum Albumin Protein	Acro Biosystems	Cat# HSA-H82E3
Chromium Next GEM Chip K Single Cell Kit	10X Genomics	PN# 1000287
Chromium Single Cell V(D)J Amplification Kit, Human BCR	10X Genomics	PN# 1000255
Chromium 5’ Feature Barcode Kit	10X Genomics	PN# 1000256
Chromium Next GEM Single Cell 5’ Gel Bead Kit v3	10X Genomics	PN# 1000264
Chromium Next GEM Single Cell 5’ Library Construction Kit	10X Genomics	PN# 1000190
96-well Skirted PCR plates White Barcoded	Bio-Rad	Cat# HSP9901
FACSMelody Cell sorter	BD Biosciences	N/A
Chromium Controller	10X Genomics	N/A
NEBuilder HiFi DNA Assembly Master Mix	New England Biolabs	E2621X
NovaSeq 6000 Sequencing System	Illumina	N/A
NovaSeq 6000 S4 Reagent Kit v1.5	Illumina	20028313
NovaSeq 6000 S2 Reagent Kit v1.5	Illumina	20028316
NovaSeq 6000 S1 Reagent Kit v1.5	Illumina	20028319
NovaSeq 6000 SP Reagent Kit v1.5	Illumina	20028401
Glomax reader	Turner BioSystems	Model# 9101-002
UltrAuFoil R 1.2/1.3 grids (300-mesh)	Quantifoil Micro Tools GmbH	N/A
Strep-TactinXT Superflow high capacity resin	IBA Life Sciences	Cat# 2-4010-010
NanoDrop 2000C	Thermo Scientific	Cat# ND-2000C
Prometheus NT.48 NanoDSF	NanoTemper Technologies	N/A
Leica DMi1 Inverted Microscope	Leica Microsystems	N/A
Tecnai F20 electron microscope	FEI	N/A
TemCam F415 CMOS camera	TVIPS	N/A
Vitrobot mark IV	Thermo Scientific	N/A
Solarus 950 plasma system	Gatan	N/A
PELCO easiGlow	Ted Pella Inc.	N/A
Talos Arctica	Thermo Scientific
FEI Titan Krios	Thermo Scientific	N/A
K2 Summit direct electron detector camera	Gatan	N/A
C18 ZipTip	Merck Milipore	Cat# ZTC18S008
Vivaspin 500, 3 kDa MWCO, Polyethersulfone	Sigma-Aldrich	Cat# GE28-9322-18
Orbitrap Eclipse mass spectrometer	Thermo Fisher Scientific	N/A
Ultimate 3000 HPLC	Thermo Fisher Scientific	N/A
EasySpray PepMap RSLC C18 column (75 μm x 75 cm)	Thermo Fisher Scientific	Cat# ES805
PepMap 100 C18 3μM 75μM x 2cm	Thermo Fisher Scientific	Cat# 164946
NEXTERION Slide H NHS-ester activated glass slides	Schott Inc.	SKU:1070936
sciFLEXARRAYER S3 non-contact microarray	Scienion Inc.	N/A
Scienion PDC80 nozzle	Scienion Inc.	N/A
GenePix 4000B microarray scanner	Molecular Devices	N/A