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. 2023 Mar 7;120(11):e2300605120. doi: 10.1073/pnas.2300605120

Fig. 3.

Fig. 3.

Staggered cutting by vCas9 suppresses NHEJ and makes MMEJ or HDR dominant. (A) DNA break positions in the target strand (TS) and nontarget strand (NTS) for engineered Cas9 variants. (B) Distributions of indel sizes induced. (C) Mean indel size induced at several loci. (D and E) Frequencies of repair pathways engaged (D) without (noT) and (E) with (T) a repair template at several loci. (F) Degree of depletion by a repair template for indels of different sizes averaged over several loci. The fraction depleted is increased by vCas9 at all indel sizes. *indicates P < 0.05 for indel size compared to wild-type Cas9 in B and C, for NHEJ frequency compared to wild-type Cas9 in D and E, or for indel depletion compared to wild-type Cas9 in F. Data were analyzed by deep sequencing and represent means of n = 3 independent replicates with SEs.