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. 2023 Mar 7;120(11):e2300605120. doi: 10.1073/pnas.2300605120

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Copyright © 2023 the Author(s). Published by PNAS.

This article is distributed under Creative Commons Attribution-NonCommercial-NoDerivatives License 4.0 (CC BY-NC-ND).

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Fig. 4. — vCas9 enhances precise editing and suppresses indels across diverse editing contexts. (A) Precise editing and indel frequencies using small replacement (<10 bp) HDR templates at several loci. (B) Frequency of precise gene conversion from GFP to BFP. (C) Flow cytometry plots showing BFP-positive (indicating precise edits), nonfluorescent (indicating indels), and GFP-positive (indicating unedited cells) cell population fractions. (D) Design of a gRNA and HDR template to introduce a large insertion. (E) Design of a gRNA to induce untemplated collapse of duplications. (F) Precise editing and indel frequencies using large insertion (~50 bp) templates at several loci. (G) Precise editing and indel frequencies for untemplated collapse of duplications (10 to 20 bp) at several loci. *indicates P < 0.05 for precise editing frequency compared to wild-type Cas9. Data were analyzed by deep sequencing in A, F, and G or flow cytometry in B and C and represent means of n = 3 independent replicates with SEs.