Table 2. Susceptibility to DcpS and Nudt16 and Capping Efficiency in IVT.
| no. | compound | DcpS (% of hydrolysis after 30 min) | Nudt16 (% of hydrolysis after 60 min) | capping %a |
|---|---|---|---|---|
| m7,2′OGpppG | n.d. | n.d. | 67 | |
| m7GpppG | hydrolyzed (69%) | hydrolyzed (79%) | ||
| 1 | m7GpppG8Br | hydrolyzed (88%) | resistant | 52 |
| 2 | m7,2′OGpppG8Br | resistant | resistant | 12 |
| 3a | m7GpppG8Py | hydrolyzed (98%) | hydrolyzed (36%) | 30 |
| 3b | m7GpppG8Ph | hydrolyzed (47%) | resistant | 45 |
| 3c | m7GpppG8DMAPh | hydrolyzed (71%) | resistant | 30 |
| 4a | m7,2′OGpppG8Py | resistant | hydrolyzed (30%) | 64 |
| 4b | m7,2′OGpppG8Ph | resistant | resistant | 16 |
| 4d | m7GpppG8PhCN | hydrolyzed (25%) | resistant | 20 |
| 4e | m7,2′OGpppG8Me | hydrolyzed (27%) | resistant | 50 |
| 5b | 8Phm7,2′OGpppG | resistant | hydrolyzed (98%) | 47 |
| 5c | 8DMAPhm7,2′OGpppG | resistant | hydrolyzed (80%) | 38 |
| 5d | 8PhCNm7,2′OGpppG | resistant | hydrolyzed (62%) | 46 |
| 5e | 8Mem7,2′OGpppG | resistant | hydrolyzed (99%) | 55 |
| 5f | 8cPrm7,2′OGpppG | resistant | hydrolyzed (94%) | 52 |
a
Fraction of capped RNA