Fig. 4.

T lymphocyte-derived tissue sEV miR-142-3p promotes thyrocyte destruction. A Colony formation assays showed that miR-142-3p suppressed thyrocyte cell proliferation. B Flow cytometry suggested that mimic-treated thyrocytes underwent a significant increase in apoptosis, whereas the inhibition of miR-142-3p decreased the apoptosis of thyrocytes. C Mimic-treated thyrocyte cells can secrete more CXCL9/11, whereas the inhibition of miR-142-3p decreased the secretion of CXCL9/11. D The size of the organoid decreased after transfection with the miR-142-3p mimics and increased after transfection with the inhibitor; scale bar, 100 μm. E Apoptosis was increased in organoids after transfection with the mimics and decreased after transfection with the inhibitor. F The chemokine assay showed that mimic-treated thyroid organoids secreted more CXCL9/11. In contrast, the miR-142-3p inhibitor decreased the secretion of CXCL9/11