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. 2023 Jun 6;21:53. doi: 10.1186/s12969-023-00833-8

Fig. 3.

Fig. 3

Transfection with miR-27a-3p enhances FLS cytokine secretion. FLS were transfected with either miR-27a-3p mimic or miR-NC, then stimulated with pooled JIA synovial fluid (SF), or were left unstimulated. A Expression of the indicated cytokine genes was quantified using RT-qPCR, normalized to reference genes HPRT1 and GAPDH. Graphs show individual paired samples (n = 8). B Secretion of IL-6, CXCL8/IL-8, and CCL2 protein in culture supernatants was quantified by ELISA. Graphs show individual paired samples (n = 6–8). *p < 0.05