Fig. 5.

Overexpression of miR-27a-3p modulates the expression of genes in the TGF-β pathway in FLS. FLS were transfected with miR-27a-3p mimic or miR-NC and cultured with or without pooled JIA synovial fluid (SF). Gene expression was quantified by qPCR array and the data were normalized to three reference genes as described in Methods. The heat map illustrates the fold-change in mRNA expression in FLS cells overexpressing miR-27a-3p relative to miR-NC without stimulation (left) or with SF stimulation (right) (n = 6). *p < 0.05