Figure 1.

Consistency of peptide chromatographic peak areas, retention times, and identifications from nano-LC-MS analysis when samples were prepared with the detergent-free one-pot rapid digestion protocol. RBCs were spotted and counted in OP384 and processed using a detergent-free one-pot rapid digestion described herein. The input number of RBCs ranged from 0 to 8 RBCs with 0 RBCs serving as a background control. The X-axes denote the number of RBCs input. Panels A, B, and C depict peak areas and total ion chromatogram (TIC) normalized peak areas for trypsin and Lys-C derived HSA, HBB, and HBA peptides, respectively, and across all replicates. Panel D depicts peptide retention times for HSA (left), HBB (middle), and HBA (right) across all replicates; human serum albumin (HSA), hemoglobin beta subunit (HBB), hemoglobin alpha subunit (HBA).