Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2023 Jun 4;14(1):2215602. doi: 10.1080/21505594.2023.2215602

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

This work was authored as part of the Contributor’s official duties as an Employee of the United States Government and is therefore a work of the United States Government. In accordance with 17 USC 105, no copyright protection is available for such works under US Law.

This is an Open Access article that has been identified as being free of known restrictions under copyright law, including all related and neighboring rights (https://creativecommons.org/publicdomain/mark/1.0/). You can copy, modify, distribute, and perform the work, even for commercial purposes, all without asking permission.

PMC Copyright notice

Figure 5. — Partial deletion mutation in porin locus in patient 2B: (a) Schematic view of the Porin locus in patient 2B isolates. The first four isolates from patient 2B (2B–1 to 2B–4) had two intact copies of the porin gene; the last seven isolates (2B–5 to 2B–11) had a ~ 140bp deletion in the 5’end of the first porin paralog and a 489 bp deletion upstream. (b) PCR amplification of the porin locus. Analysis of the PCR products of patient 2B and Seattle outbreak isolates by gel electrophoresis showed a ~ 1.5kb for isolates with the intact locus and ~ 0.6 kb band for porin mutant strains. (c) Porin protein expression Protein extracts from 2B isolates were resolved by SDS-PAGE followed by western blot using a polyclonal anti-Porin (MspA) antibody. Isolate 2B–5 through 2B–11 exhibited a significant reduction in porin protein expression.