Extended data Fig. 5: Lack of Ch25h in IECs and cholesterol uptake inhibition control CD98 expression IgA secreting plasma cells in the duodenum.

a, Number of CD98^hi and CD98^lo PCs and total IgA+PC in lamina propria of mice treated with 10mg/kg (body weight) of EZT or vehicle and euthanized 24 hours later. b, Representative flow cytometry and frequency and total number of IgA⁺ PCs in the ileum of IEC^Wt and IEC^iΔCh25h mice (c). d, Representative flow cytometry and compiled quantification of frequency and total number of IgA⁺ PCs in the ileum of mice treated for 24 hours with NF, HCF and EZT (e). f, Representative flow cytometry and frequency of IgA⁺PCs in duodenum lamina propria of mice treated with EZT or Mevastatin or vehicle and euthanized 24 hours later. g, Flow cytometry and frequency of IgA⁺ PCs in BM of mice treated with EZT and Mevastatin as in (f). The results are pooled from three independent experiments (a)(n=5 mice per group); (b and c)(n=7 mice per group) and two independent experiments in (d,e,f and g)(n=4 mice per group). Statistics were measured as two-sided unpaired Student’s t-test (**p<0.01,***p<0.001) in (a,b and c) and one-way ANOVA with Bonferroni’s correction (***p<0.001) in (d,e,f and g). Exact P values and adjustments are provided in Source data.The error bars represent the mean ± s.e.m.