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. Author manuscript; available in PMC: 2023 Jun 6.
Published in final edited form as: Nat Immunol. 2023 Jan 19;24(3):531–544. doi: 10.1038/s41590-022-01413-w

Figure 2: Diet-dependent, IEC-derived oxysterols modulate IgA secretion in the small intestine.

Figure 2:

a, M12 migration assay with lipid extracts of IECs from VillincreCh25hfl/fl (IECΔCh25h) and littermate controls (IECWt) treated with NF and 2% of cholesterol diet (HCF). b, Relative 7α,25-HC and 25-HC quantification (c) in lamina propria (LP) and Lymph of IECΔCh25h and littermate mice upon NF and 2% HCF. d, Representative IgA ELISPOT and compiled quantification of IgA secreting PCs in lamina propria of IECWt and IECΔCh25h mice fed for 1 week with NF or HCF. e, ELISA of IgA in intestinal lavages and serum of IECWt and IECΔCh25h mice treated as in (d). f, Representative IgA ELISPOT and compiled quantification of IgA secreting PCs in lamina propria of IECΔCh25h and littermate control mice treated with 10 mg/kg of Ezetimibe (EZT) or vehicle (DMSO) for 24 hours. g, Analysis of the IgA+coated and non-coated commensal bacteria in the intestinal lumen of IECWt and IECΔCh25h mice by 16S rRNAseq. The results are pooled from three independent experiments (a,b,c)(n=8–12 mice per group); (d)(n=7–8 mice per group);(g)(n=3 mice per group) and two independent experiments (e,f)(n=5 mice per group). Statistic were measured with two-way ANOVA with Bonferroni’s correction (*p<0.05, **p<0.01, ***p<0.001, ****p<0.0001) in (a,b,c,d and e) and two-sided unpaired Student’s t-test (****p<0.0001) in (f). Exact P values and adjustments are provided in Source data. The error bars represent the mean ± s.e.m.