Figure 5.

sEVs miR-503 targets endothelial IGF1R and inhibits IGF1R expression (A) Potential targets of miR-503 were predicted using miRDIP (https://ophid.utoronto.ca/mirDIP/) and predicted targets with high scores were applied for the Kyoto Encyclopedia of Genes and Genomes (KEGG) signaling pathway enrichment annotation. (B) HUVECs were transfected with miR-503 mimics or miR-503 inhibitors and examined for the protein levels of IGF1R were examined using Immunoblotting. (C) Wild- and mutant-type IGF1R 3’UTR luciferase reporter vectors were constructed and co-transduced into tool cells (293T) with miR-503 mimics or inhibitor. Luciferase activity was determined. N=3, ** p<0.01 vs. mimics NC group. ## p<0.01 vs. inhibitor NC group.