Figure 2.

Identification of VAPB and SERCA2 as putative PERK interactors. HEK293-T cells were transfected with myc-tagged expression vectors for PERK WT (PERK^WT) and PERK Kinase dead (PERK^KD). After 48 h of transfection, PERK^WT and PERK^KD were immunoprecipitated (PERK IP) and interactors were detected using antibodies against VAPB and SERCA2 by immunoblot. Input was 10% of the total protein amount used for the IP (50 µg of protein was loaded as an input versus 500 µg of total protein used for the IP). Unspecific anti-mouse antibody was used as a negative control (Mouse IP). Data show are representative of N = 3 (VAPB), N = 2 (SERCA2) biologically independent experiments.