Figure 2.

Opn3/OPN4 RGCs constitute the largest proportion of multi-opsin RGCs (A–J). Whole-mount retinae from P28 Opn3-eGFP; Opn5^cre; Ai14 mice imaged for GFP (green), OPN4 (blue), and tdTomato (red). Both low (A, F, 50 µM scale bars) and high (B, G) magnifications are shown as are the channel separations (C–E and H–J). K: Venn diagram showing the amount of RGCs, out of 3306 assessed, that express, single, double, or triple combinations of Opn3, OPN4, and Opn5. These data are also represented in the chart (L) showing the opsin expression combinations as a proportion of a single class of opsin-expressing RGCs.