Table 1. Genotyping primers.
| Allele | Primers | Pairs | bp |
|---|---|---|---|
| Opn3 |
F1: ACCCAGGCTTCTTTTGGTCT |
F1R1 – Wild-type |
1191 |
| |
R1: AGAGTCGTTGGCATCCTTGG |
F1R1 – Opn3fl |
1231 |
| |
F2: ACTATCCCGACCGCCTTACT |
F1R2 – Opn3fl |
1610 |
| |
R2: GAACTGATGGCGAGCTCAGA |
F1R2 – Opn3cko |
640 |
| Opn3ΔEx2 |
F1: CTCAGAACCCACAAAGTGCTGG |
F1R1 – Wild-type |
307 |
| |
R1: GTGGACTGCAATGTCCCATCTATC |
F1R2 – Opn3ΔEx2 |
191 |
| Opn3Tg(Opn3-EGFP)JY3Gsat |
F1: CAGAGCGTGAGATCCACCCTGTT |
F1R1 – Opn3eGFP |
320 |
| R1: TAGCGGCTGAAGCACTGCA |