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. 2023 Apr 24;8(8):e166643. doi: 10.1172/jci.insight.166643

Figure 3. ABT-263 and FST improve renal function and tubular repair in post-RLDC mice.

Figure 3

C57BL/6 mice were subjected to RLDC treatment followed by 4-week treatment of ABT-263 (n = 13), FST (n = 8), or vehicle (n = 14). Control (CT) mice (n = 7) were injected with saline. Blood and kidney samples were harvested at 8 weeks for renal function and histology analysis. GFR was measured 1 day before tissue harvesting. (A–D) GFR, BUN, SCr, and kidney weight to body weight ratio (KW/BW). (E) Representative images of VIM staining. Scale bar: 50 μm. (F) Quantification of VIM staining. Positive signals from blood vessels and glomeruli were excluded. (G) Representative images of costaining of Ki67 (red) and LTL (green). The bottom images are enlarged from the boxed areas in the top images. Original magnification, ×200 (original); ×400 (enlarged). Arrows indicate regenerative tubules positive for LTL and with Ki67-positive nuclei. Scale bar: 50 μm. (H) Quantification of LTL-positive staining areas. (I) Quantification of Ki67+/LTL+ regenerative tubules. Quantitative data are presented as mean ± SD. One-way ANOVA with multiple comparisons was used for statistics. *P < 0.05, **P < 0.01, ***P < 0.005, and ****P < 0.001.