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. 2023 Apr 24;8(8):e166643. doi: 10.1172/jci.insight.166643

Figure 4. RLDC induces senescence in cultured mouse renal proximal tubular (BUMPT) cells.

Figure 4

BUMPT cells were subjected to RLDC treatment or kept in control (CT) medium for 96 hours. Cells were collected for senescence analysis. (A) Cell morphology was recorded by phase contrast microscopy. Scale bar: 200 μm. (B and C) Quantification of the numbers and sizes of BUMPT cells (n = 4 experiments). (D) Representative images of costaining of SA-β-gal (top panels) and DAPI (bottom panels). Scale bar: 50 μm. (E) Quantification of SA-β-gal–positive cells (n = 5 experiments). (F) Immunoblot analysis for P53, P21, P16, and BCL-XL. (G–J) Densitometry of P53, P21, P16, and BCL-XL expression (n = 7 experiments). (K–S) RT-PCR analysis of p16, p19, p21, Ctgf, Fgf2, Pdgf, Tgfb, Il-6, and Tnfa mRNA expression (n = 5 experiments). Quantitative data are presented as mean ± SD. Two-tailed, unpaired t test was used for statistics. *P < 0.05, **P < 0.01, ***P < 0.005, and ****P < 0.001.